Mass Spectrometry of Proteins and Archeology
نویسندگان
چکیده
منابع مشابه
Mass Spectrometry of Amino Acids and Proteins
Likemostmatter (with the exception of, say, neutron stars), proteins and peptides are mostly made of nothing – an ephemeral cloud of electrons with very little mass surrounding tiny and very dense atomic nuclei that contain nearly all of themass (i.e., peptides and proteins aremade of atoms). Atoms havemass and the unit ofmass that is most convenient to use is called the atomic mass unit (abbre...
متن کاملMass spectrometry of peptides and proteins.
This tutorial article introduces mass spectrometry (MS) for peptide fragmentation and protein identification. The current approaches being used for protein identification include top-down and bottom-up sequencing. Top-down sequencing, a relatively new approach that involves fragmenting intact proteins directly, is briefly introduced. Bottom-up sequencing, a traditional approach that fragments p...
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Quantitative measurement of proteins is one of the most fundamental analytical tasks in a biochemistry laboratory, but widely used immunochemical methods often have limited specificity and high measurement variation. In this review, we discuss applications of multiple-reaction monitoring (MRM) mass spectrometry, which allows sensitive, precise quantitative analyses of peptides and the proteins ...
متن کاملDesorption electrospray ionization-mass spectrometry of proteins.
Desorption electrospray ionization-mass spectrometry (DESI-MS) was evaluated for the detection of proteins ranging in molecular mass from 12 to 66 kDa. Proteins were uniformly deposited on a solid surface without pretreatment and analyzed with a DESI source coupled to a quadrupole ion trap mass spectrometer. DESI-MS parameters optimized for protein detection included solvent flow rate, temperat...
متن کاملSecondary Ion Mass Spectrometry of Proteins
The adsorption of bovine serum albumin (BSA), immunoglobulin G (IgG) and fibrinogen (Fgn) on fluorinated selfassembled monolayers have been studied using time of flight secondary ion mass spectrometry (ToF-SIMS) and Spectroscopic Ellipsometry (SE). The objective of the work has to establish the utility of ToF-SIMS for the determination of the amount of protein adsorbed on the surface. Quantific...
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ژورنال
عنوان ژورنال: Journal of the Mass Spectrometry Society of Japan
سال: 2018
ISSN: 1340-8097,1880-4225,1884-3271
DOI: 10.5702/massspec.s18-43